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spr sensograms  (Biacore)
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Biacore spr sensograms
Spr Sensograms, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spr sensograms and equilibrium curves  (GraphPad Software Inc)
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Spr Sensograms And Equilibrium Curves, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spr sensograms  (Danaher Inc)
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The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) <t>SPR</t> <t>sensograms</t> for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.
Spr Sensograms, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+sensograms/pmc10533329-261-0-10?v=Danaher+Inc
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biacore spr sensograms  (Biacore)
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The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) <t>SPR</t> <t>sensograms</t> for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.
Biacore Spr Sensograms, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+sensograms/pm37753968-188-0-1?v=Biacore
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surface plasmon resonance (spr) sensogram  (Biacore)
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Biacore surface plasmon resonance (spr) sensogram
The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) <t>SPR</t> <t>sensograms</t> for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.
Surface Plasmon Resonance (Spr) Sensogram, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spr sensograms res pte pic rhaprp23-231  (BioCore Inc)
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BioCore Inc spr sensograms res pte pic rhaprp23-231
The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) <t>SPR</t> <t>sensograms</t> for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.
Spr Sensograms Res Pte Pic Rhaprp23 231, supplied by BioCore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+sensograms/pm32511904-224-8-11?v=BioCore+Inc
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spr sensograms res pte pic rhaprp23-231 - by Bioz Stars, 2026-07
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spr-sensograms  (Biacore)
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Biacore spr-sensograms
The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) <t>SPR</t> <t>sensograms</t> for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.
Spr Sensograms, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+sensograms/pm29674419-205-1-6?v=Biacore
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The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) SPR sensograms for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.

Journal: The Plant Cell

Article Title: Allelic compatibility in plant immune receptors facilitates engineering of new effector recognition specificities

doi: 10.1093/plcell/koad204

Figure Lengend Snippet: The RGA5 APB mutant binds and recognizes AVR-Pik when integrated into Pikm-1. A) Pikm-1 APB chimera responds to all variants of AVR-Pik tested and activates cell death when coexpressed with Pikp-2 in N. benthamiana , but like Pikm-1 RGA5 , it only weakly responds to AVR-Pia. B) Cell death scoring of Pikm-1 APB coexpressed with AVR-Pik variants D, C, and F in N. benthamiana represented as dot plots. The total number of repeats was 80 per sample. For each sample, all the data points are represented as dots with a distinct color for each of the 3 biological replicates; these dots are jittered around the cell death score for visualization purposes. The size of the central dot at each cell death value is proportional to the number of replicates of the sample with that score. Statistical analyses of these results are shown in . C) co-IP of Pikm-1 APB with different MAX ( Magnaporthe AVRs and ToxB-like) effectors shows association with AVR-Pik variants, but not AVR-Pia, in planta. Dotted line denotes separate membrane exposures of the same membrane. D) SPR sensograms for the interaction of HMA domains of Pikm-1, RGA5, and RGA5 APB mutant with effectors AVR-PikD, AVR-PikC, AVR-PikF, and AVR-Pia. Non-MAX effector AVR-Pii was added as a negative control. RUs for each labeled protein concentration are shown with the residuals plot beneath (SPR acceptance guides as determined by Biacore software are shown as green and red lines in the residuals plots). Concentration of each protein in the assay is indicated next to their corresponding name. Each experiment was repeated a minimum of 3 times, with similar results.

Article Snippet: SPR sensograms were analyzed with the Biacore Insight Evaluation Software (Cytiva) and equilibrium dissociation constants ( K D ) values were calculated using a 1:1 binding model from a kinetic fit model.

Techniques: Mutagenesis, Co-Immunoprecipitation Assay, Membrane, Negative Control, Labeling, Protein Concentration, Software, Concentration Assay